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We can easily observe that freight transport operations have become more and more complex and differentiated over the past 30 years.
Therefore, to determine the actual magnitude of change or probabilities, the marginal effect of the explanatory variables, Equation 1 is differentiated over the explanatory variables to give Equation (2): ∂ P y = jlx ∂ x k = P y = jlx β ik - ∑ i ≥ 1 β ik exp Xβi 1 + ∑ i ≥ 1 exp Xβi (2).
Therefore, we suggest that the two OsPORs have differentiated over the course of evolution, playing distinct roles in the adaptation of rice to the environment.
To understand the time course of transcriptional changes occurring in these cells, we performed RT-PCR analysis of hESC-derived EBs as they differentiated over the course of 42 days into beating clusters (Figure 1b).
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Pluto TV, a video streaming service targeting cord cutters, has differentiated itself from other over-the-top competitors like Netflix by offering a more TV-like experience with over 100 "channels" you flip through in an interface that looks similar to a traditional TV guide.
The neurospheres remained differentiated neurons over the two-week cultivation in the multi-organ chip, proven by qPCR and immunofluorescence of the neuronal markers βIII-tubulin and microtubule-associated protein-2.
Future studies should evaluate the effect of both factors in the modulation of geno- and phenotype of the differentiated cells over the time and study the underlining mechanism.
When BrdU was added to the differentiated cells over the entire 5 days of the hepatic endoderm generation stage, less than 5% of the AFP+ hepatic endoderm cells demonstrated BrdU incorporation (Figure 2C).
Although it is reassuring that survival has improved with poorly differentiated tumours over the years, these findings are not evident in stage II disease and clear cell histology.
We propose two linked hypotheses: first, when Ptf1a is lost from individual cells, other acinar-specific transcription factors that prevent reprogramming and co-regulate PTF1 target genes, such as NR5A2 and BHLHA15/MIST1, are sufficient to maintain a differentiated phenotype over the short term.
The cells were differentiated over 6 days using 20 40% L929 supernatant (M-CSF producing murine fibroblast cell line) in RPMI (Lonza) supplemented with 10% heat-inactivated FCS (Sigma-Aldrich), 100 U ml−1 penicillin, 100 μg ml−1 streptomycin, and 292 µg ml−1 l-glutamine (Gibco).
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