Immunohistochemical assays were performed on lymphosarcomas for evaluation of lymphocytes lineage and on spindle cells tumors with poorly differentiated morphology for determination of tissue of origin.
This was accompanied by the acquisition of a differentiated morphology, all of which characteristics were maintained as long as N.1 cells were exposed to the inducer.
Interestingly, these polyps retained relatively well-differentiated morphologies of adenomatous polyps and did not show any increase in invasive properties.
Although we also observed moderately expressed miR-203 in tumor regions with evidence of cellular differentiation (keratin pearls and large differentiated morphology), the levels of miR-203 was considerably lower in these regions compared to the suprabasal cells of adjacent normal skin, where miR-203 is normally expressed.
The Sr-doped granules exhibited the highest proliferation yields and efficiency in osteoblastic maturation, including acquisition of a differentiated morphology and high levels of secreted alkaline phosphatase.
We also used the SV40 large T antigen transformed 16HBE cell line, referred to as "non-tumor cells" or "non-tumor cell line," a human bronchial epithelial cell line that retains the differentiated morphology and function of normal human airway epithelium [26].
16HBE 14o-cells, a simian virus 40 large T antigen transformed human bronchial epithelial cell line that retains the differentiated morphology and function of normal human bronchial epithelia [ 22], were a kind gift from Dr. D. Gruenert (California Pacific Medical Center, CA, USA).
Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells, and a significant increase in myosin-positive and multinuclear cells.
A proportion of tumor cells are slow dividing, have a less differentiated morphology and are capable of giving rise to metastasis more efficiently.
Selenium supplementation in the SH-SY5Y cells also induced a differentiated morphology and changed expression of the adhesion protein fibronectin 1 and the differentiation marker cadherin 11, as well as different temporal expression of the studied TXNRD1 variants.
