Exact(3)
The cell aggregates were then suspension-cultured in ultra-low attachment cell culture dishes with hESC differentiated medium (without basic fibroblast growth factor) for 9 days with the media changed every two days.
At the same time, neonatal CMCs were grown on a p24w plate with a differentiated medium for 0 4 days.
Histological grade is defined by the SEER Program Coding Manual and by the International Classification of Diseases for Oncology, Second Edition in increasing level of severity as either low grade (well differentiated), medium grade (moderately differentiated), or high grade (poorly differentiated) [ 15, 19].
Similar(57)
MuSCs were differentiated in differentiation medium (DMEM (Invitrogen) with 2% horse serum (Sigma)).
Two days after plating, cells were differentiated using "Differentiation Medium" (catalog # DM-2, ZenBio) and considered as Day '0'.
To improve the efficiency of node cooperation and multiple access performance in multihop wireless networks, a rapid cooperation-differentiated medium access control (MAC) protocol is proposed.
Whole-cell perforated patch recordings were performed from sorted HUES-9 NSC that were differentiated in neuron differentiation medium for 3 weeks.
After rADSCs were differentiated with endothelial differentiation medium for 8 days, the cells were trypsinized and seeded in a 24-well plate coated with Matrigel for angiogenesis assay.
Based on western blot analysis, the protein expression of vWF was also detected after rADSCs were differentiated with endothelial differentiation medium for 8 days.
After rADSCs were differentiated with endothelial differentiation medium for 8 days, the cells were trypsinized and seeded a 24-well plate which was coated with Matrigel (8.8 mg/ml; BD,USA) at a concentration of 5 × 10/well in endothelial differentiation medium.
To characterize the cells, lnASCs or obASCs transfected with the leptin shRNA or control shRNA were differentiated in osteogenic differentiation medium or adipogenic differentiation medium, plated to determine colony forming unit capacity, and analyzed for the expression of cell surface markers.
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