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They are also opening offices in Silicon Valley and working closely with companies like Bubble Motion and Placecast, which enable operators to get a slice of that $22 billion pie all through a differentiated media offering.
The following day, the medium was replaced during 14 days by any of the differentiated media DifM 1 4.
After EB formation, 4-day EBs were cultured with various differentiated media (Table 1) for 2 weeks.
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To examine the effects of CA extracts on differentiation of pre-adipocytes to adipocytes, cells were differentiated with differentiation media containing various concentrations of the plant extract.
At confluence (day 0), preadipocytes were differentiated in differentiation media (DM) for 72 h (day 03) and were maintained in nutrition media (NM) thereafter for 11 days (day 14).
Fifty thousand cells per well were seeded on Seahorse plate and differentiated in differentiation media containing 20% FBS, 20 n m insulin and 1 n m triiodothyronine for 48 h, than were supplemented with 0.5 m m dexamethasone, 0.5 m m isobutylmethylxanthine, and 0.125 m m indomethacin for 48 h, and returned to differentiation media.
After 5 days in culture, the cells were seeded at a density of 1 × 10 per well and differentiated to osteoblasts in differentiation media (50%F-12F-120% DMEM, 0.1% L-Glutamine, 0.1% penicillin/streptomycin, 10 mM β-glycerophosphate, 100 nM dexamethasone, 0.2 mM ascorbic acid (Reyes et al, 2001) in the presence or absence of 200 nM 4-OH-Tamoxifen, and the medium was changed every 2 days.
The medium was changed every other day and cultures were differentiated with the addition of differentiation media when they reached 60 70 % confluency.
Cells were differentiated by culturing in Osteoblast Differentiation media (LONZA UK) for 15 days.
Non-proliferating, polynucleated, fully differentiated myotubes were obtained after seven days in differentiation media.
CD34+ cells were expanded and differentiated to monocytes in two-step cultures in the expansion and differentiation media, each step 7 10 days, as previously described [ 17].
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