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In the superficial differentiated layer of the urothelium, the umbrella cells, high CK8 expression was observed.
Unlike these cells, the syncytiotrophoblast, the outer differentiated layer of placental villi, appeared completely negative for this protein.
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The comparison of our data with the transcriptional profile of the genes that are differentially expressed in the basal and differentiated layers of the epidermis suggests that TGF-β/phospho-Smad pathway-induced transcription profile fades along the epidermal differentiation axis.
TLR9 was also expressed in the differentiated layers of HPV-positive cervical epithelial neoplasias (Fig. S3).
As shown in Figure 1D, HA staining was evident in the differentiated layers of the skin epithelium, but not in the undifferentiated basal layers.
Although the Ets1 transgene was expressed in the more differentiated layers of the epidermis (upper spinous and granular layers), the PCNA and Ki67 labeling was mainly associated with less differentiated layers (basal and lower spinous layers).
Based on these criteria, the differentiated layers of the epidermis can be visualized as three separate regions: the spinous or prickle cell layer (stratum spinosum), the granular layer (stratum granulosum) and the cornified layer (stratum corneum).
Interestingly expression of c-myc in the differentiated layers of the skin also results in hyperproliferation and formation of papillomas [57], whereas targeting c-myc to the basal proliferative layer does not promote keratinocyte proliferation or papilloma formation [58].
To confirm this result, we stained sections of the dorsal skin of BT and control mice with antibodies specific for marker proteins associated with particular differentiated layers of the epidermis.
The majority of the studies reported herein were performed using the transgenic line C, but similar results were obtained with line E. To determine whether the transgene was faithfully expressed in the differentiated layers of the epidermis, immunohistochemical analysis using anti-HA antibodies was carried out.
Of note, we did not observe any defects in the formation of the differentiated layers of the skin in the miR-203 KO mice.
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