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For instance, the plumage variation may have appeared recently and differentiated independently in each expanding clade.
The results from this study confirm the hypothesis that R. sativus and the three Brassica diploids differentiated independently from a common ancestor with A. thaliana and that this divergence occurred via genome rearrangement and/or polyploidy events at and/or after differentiation.
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In mice, the selective ablation of oestrogen receptor in mature osteoclasts mimicked an osteoporotic bone phenotype [ 24], suggesting that oestrogen directly regulates the life span of differentiated osteoclasts, independently of an inflammatory pathway.
For mRNA and functional validations of the gene expression profiles, we then used 3 13 independently differentiated hiPSCs-astrocyte cultures from three healthy controls and two SOD1D90A ALS patients.
To study whether Cdc42 regulates the maintenance of apical structures of fully differentiated supporting cells, independently of its maturational role, mice were treated with tamoxifen between P16 and P18, and the analysis was performed at P25.
We validated five significantly altered transcripts belonging to EphB1 and/or STAT3 networks by qPCR using eight and seven independently differentiated hiPSCs-astrocyte cultures from three healthy controls and two SOD1D90A ALS patients, respectively (Fig. 7a; Supplementary Table 1 and 2).
Each macrophage population was independently differentiated, cultured and stimulated before analysing the biological material.
Common stroke signs in young patients such as headache, leg paresis and diplopia were seldom or not independently differentiated from other stroke signs.
N denotes the number of individual cell lines and n describes the total number of independently differentiated batches of hCNs used as the statistical n for each experiment (the number of independent observations).
We further included a locally adapted and genetically differentiated (i.e., independently evolving) ecotype from the P. mexicana mexicana clade: the hydrogen sulfide-adapted form inhabiting El Azufre, a tributary to the Río Oxolotan.
These two types of primitive AHLs duplicated, differentiated and further developed independently into members of Type-I and -II AHLs in early land plants, defining the two clades (Clade-A and -B).
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