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Mature cystic teratoma (MCT) is the most common neoplasm of the ovary and includes at least two well- differentiated germ cell layers.
10.7554/eLife.08174.008 Figure 4. Wnt signaling in ISCs prevents BMP signaling in the differentiated germ cell zone and maintains long ISC cellular processes.
We triple immunostained xenografts for OCT3/4 (undifferentiated germ cell marker), MAGE-A4 (differentiated germ cell marker), and the proliferation marker Ki67.
This is consistent with SSE being derived from a more differentiated germ cell in the spermatogenic lineage than SE or NS (Verkerk et al, 1997).
This is an important observation as many of the initial samples with YCMD cluster in groups distinct from their most differentiated germ cell type.
Accurate determination of testicular developmental stages was achieved by combining histological analysis of Bouin's solution fixed samples (on the basis of the most differentiated germ cell type present in the gonad) and GSI, as previously described in details [ 12, 42].
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At the beginning of life, terminally differentiated germ cells fuse to generate a totipotent stem cell, the fertilised egg.
Therefore, the terminally differentiated germ cells and highly plastic pluripotent stem cells are two critical points in the circle of life.
Therefore, it is very important to develop a suitable protocol to induce in vitro germ cell differentiation from monkey ES cells before non-human primate ES cells can be used as a model for in vitro differentiated germ cells.
We hypothesized that if the Oct4ΔPE GFP-positive pOct4ΔPE GFP-positiveof differentiated germ cells in vitro, we expopulationt loss of funconsistedDazl wofldifferentiatediminate Oct4ΔPE:germpositive cells relatine to wildtype and/or heterozygous as observitron vivo.
In agreement with the present results, in human testis, Cu/Zn SOD protein expression was shown to be intense in spermatogonia of the seminiferous tubules, while in spermatocytes and in other more differentiated germ cells and Sertoli cells, a weak expression was observed [47].
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