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The intracellular recordings were acquired from hMSCs that had been cultured and differentiated for a total of five days, whereas the voltage dye studies were conducted after a total of four weeks of culture, with differentiation being initiated at various time points within those four weeks.
Subsequently, the H9 hESC line was also differentiated for a total of 10 days.
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A wide variety of samples comprising adult and embryonic stem cells and their differentiated derivatives were collected in biological triplicate and hybridized to the Affymetrix MOE430 GeneChip Set for a total of 270 GeneChips.
Air-liquid interface culture continued for a total of seven days (time 5, 7, 10 days) in order to produce the fully differentiated epidermal equivalents.
Look for a total return of 15%.
Repeat for a total of four times.
Repeat for a total of 15 reps.
The percentages of differentiated cells were calculated based on a total of 200 cells counted randomly for approximately 5 different fields under microscope for each experiment.
It continued at differentiated speeds until the 1970s, reaching a total volume of six million, with a partial reversal after that.
The cell fate of the rest 20% new cells is unknown, and composed by non-differentiating cells and/or glial cells (including perhaps a proportion of cells fully differentiated, as the total time required for newborn neurons to fully differentiate is extremely variable –[59]-).
Most well-differentiated thyroid cancers (WDTC) are treated with a total thyroidectomy followed by selective use of radioiodine for remnant ablation (RRA) [ 1- 6].
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