"More and more firms are thinking that if they want differentiated deal flow, they need to look outside of Silicon Valley," says Harris.
Blood leukocytes were quantified and differentiated with flow cytometry by using TruCount-Tubes according to cellular side-scatter/forward-scatter characteristics and CD45, Gr-1, and CD3 expression.
Primer and probe sequences are provided in Table S1 in Additional file 1. Leukocytes in BALF were differentiated with flow cytometry, according to their side-scatter/forward-scatter characteristics, and CD45, Gr-1, and F4-80 expression (FACSCalibur; BD, Heidelberg, Germany).
Leukocytes were extracted by meshing the lung tissue through a cell strainer (100 μm) and differentiated by flow cytometry according to their side-scatter/forward-scatter properties and CD45, Gr-1 and F4-80 expression (FACSCalibur, BD, Heidelberg, Germany).
The flows exhibit excellent textural preservation with well-developed upper spinifex and lower cumulate zones typical of differentiated komatiite flows.
Two dimensional flood inundation models capable of simulating complex spatially and temporally differentiated floodplain flows are routinely used to model and predict flooding.
The green fluorescence offers an excellent marker for monitoring the differentiated cells by flow cytometry post-transplantation. Cell density was kept between 2 and 4×106 cells per ml.
We determined the expression of classical MHC class I molecules (H2Kb and H2Db) and NKG2D ligands on ES and differentiated cells by flow cytometry (Fig. 6D).
Pairwise FST calculations show that sympatric and allopatric populations of each type are not significantly differentiated, so gene flow between sympatric and allopatric populations is a possibility.
To further delineate this activity in relation to the developmental stage, we analyzed the cell cycle profile of primary fibroblast, hiPSCs, hNPCs and terminally differentiated neurons by flow cytometry before and 24 h after IR treatment (Supplementary Figure 2).
