Expression of CENP-A appeared to increase with the grades of differentiation of HCC cells from well differentiated to poorly differentiated (Figure 2B D).
The teratomas from one Δ133p53 overexpression-derived iPSC (Ci133-5) were more maturely differentiated (Figure 4e, lower panels) than those from the other four lines for currently unknown reason.
Examination of glnB and gdh1 expression levels by qRT-PCR showed that GlnB expression was slightly higher than that of gdh1, but the differences could not be clearly differentiated (Figure 2). Figure 2 Expression of glnB and gdh1.
The melting curve profiles of 7 mutation- and polymorphism-containing plasmids were unequivocally differentiated (Figure 4B).
In the absence of FGF4 or in the presence of PD173074 all mTS cells were differentiated (Figure 4).
Also a few enlarged and infected symbiotic cells were present, proximal to the root, but intracellular bacteria were not yet elongated and differentiated (Figure S4D and S4E).
We found Chk1 to be highly expressed in stage I progenitors when compared to stage IV cells, and to steadily decrease as the cells differentiated (Figure 7A).
The effect of dynamin disruption appeared to be selective for migration, since the transfected cells survived, differentiated (Figure S7B, inset), and extended axons across the corpus callosum (Figure S7D), similar to control cells (Figure S7C).
Transcriptome-wide microarray analysis of these cultures at day 0 and day 28 (before and after differentiation) showed that the expression of the human airway basal cell signature genes was markedly suppressed as the basal cells differentiated (Figure 2F).
Transduction with both types of vector was about equivalent both in proliferating (Figure 1, No NGF) and differentiated (Figure 1, Plus NGF) cells and, based on the calculated MOI and percent confluence, about 50% as efficient for both types of vector particles as on MDTF cells.
