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Using an HA-antibody, we detected expression of CagA-HA throughout the differentiated eye epithelium (Fig. 1C).
To quantify the degree of rescue, we divided the total area of deep ElaV positive expression by the total area of the differentiated eye epithelium.
The same group later reported that expression of this dominant negative in non-proliferating, terminally differentiated eye lens fiber cells caused apoptosis and accumulation of the DNA damage markers p53 and phospho-Chk2 [28].
A post-hoc script read the binary file and differentiated eye position into velocity, allowing us to easily identify the beginning and end of saccades using a 30 degrees/second threshold.
To better understand the changes that occur in the context of development, we undertook an analysis of genome-wide CpG methylation in animals at four developmental stages [sperm, one-cell, mid-blastula transition (mbt), ∼3.5 hr post-fertilization, and 3 d post-fertilization (3dpf)] and four fully differentiated (eye, brain, heart, and liver) tissues.
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In contrast, 10%% or more methylated samples are easily differentiated by eye from the unmethylated control (Fig. 2b).
Eye position signals were differentiated to obtain eye velocity.
It would be interesting to investigate whether CXCR4 and its ligand are involved; how the MSCs are distributed and differentiated in the eye with time; when is the best time to administrate MSCs to achieve optimal efficacy.
The signal was further differentiated to obtain eye acceleration, which was smoothed with a five-point moving average filter.
Even when companies create new markets at the low end, the offerings also are clearly differentiated in the eyes of buyers.
Differentiated tissues, such as eyes, heart and viscera were removed and the remaining tissues were dissociated by dispase (1.2 U/ml).
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