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Another difference in the experimental paradigm that we used was the derivation of differentiated cells from teratoma tissue as opposed to derivation from cells differentiated exclusively in vitro.
Incidentally, M. intracellulare and M. arosiense share a similar FAME profile marked by predominance of 16:0, 10-methyl 18 0 TBSA (tuberculostearic acid) and 18 1 ω9c as major fatty acids and can not be differentiated exclusively on the basis of FAME analysis [12].
These cells expressed NS cell markers and differentiated exclusively into different subtypes of neurons, astrocytes and oligodendrocytes upon the removal of LIF/CHIR/Y, even after 60 passages.
It has been shown that, after the stress treatment, microspore plastids had differentiated exclusively into amyloplasts, accumulating starch and losing their thylakoids as well as their capacity to divide (Caredda et al. 2000).
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However, Park et al. reported that in the absence of TGF-β supplementation, MSCs on soft substrates did not differentiate exclusively to chondrocytes, but also differentiated to adipocytes, generating a heterogeneous population.
The implanted hES-MP cells gave rise to homogeneous, well-differentiated tissues exclusively of mesenchymal origin and no teratoma formation was observed.
The differentiated cells could exclusively synthesize and secrete dopamine in response to stimuli.
Newly differentiated stem cells exclusively present full-length MUC1.
Fukushima et al.[ 20] reported an increase in production of new brain cells which were almost exclusively differentiated into astrocytes in hydrocephalic rats; however the study did not distinguish between the various degrees of ventriculomegaly.
Mbd3 −/− ESCs and those expressing an RNAi construct directed against an irrelevant transcript (encoding LacZ) produced 40%50%% undifferentiated colonies when plated at clonal density in the absence of LIF for 4 days, whereas under the same conditions, wild-type cells produced almost exclusively differentiated colonies.
Furthermore, adiponectin is a secreted serum protein expressed exclusively in differentiated adipocytes.
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