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For example, GBM2-derived cellsustaineded the multilineage phenotype after serum-starvation, whereas GBM1 tumor cells differentiated efficiently to mesenchymal cells in any of the differentiation conditions used in this study.
The patent says the "invention relates to a technology where stem cells from embryonic and adult brains are isolated, propagated, and differentiated efficiently in culture to generate large numbers of nerve cells".
Under these conditions, control cells differentiated efficiently into both cardiac and skeletal muscle.
However, P19 cells mixed with P19 [BMP4] cells and treated with RA differentiated efficiently into both cardiac and skeletal muscle, indicating that RA and BMP4 could antagonize each other's inhibitory activities.
After injection, they found that the grafted cells survived in the diseased tissues and differentiated efficiently into astrocytes, with microgliosis alleviated at the transplanted sites; additionally, survival was extended, motor neuron loss was ameliorated and declines in forelimb motor capability slowed, and respiratory functions improved [ 72].
In the present study, we developed a single-medium culture method using CM from cultures of ST2 stromal cells (ST2-CM), by which EBs differentiated efficiently into HC-like cells.
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Sensor networks offer a virtual path capability to carry differentiated services efficiently across the wireless backbone.
Human myoblasts (Mb) are an attractive model for analysis of differentiation because they can be efficiently differentiated into very large, multinucleated, postmitotic myotubes (Mt) in vitro and can be compared with skeletal muscle tissue, which is largely derived from such myogenic progenitors.
They can be efficiently differentiated into neural precursor cells that can be further differentiated into neurons (including specific subtypes), astrocytes, and oligodendrocytes (Itsykson et al., 2005; Lappalainen et al., 2010; Sundberg et al., 2010).
ESCs, derived from the inner cell mass of the blastocyst [ 77], can be efficiently differentiated into any cell type both in vitro and in vivo, and these differentiated cells present morphological, biochemical, and physiological traits similar to their in vivo counterparts.
Little information is available on the ability of terminally differentiated cells to efficiently repair DNA DSBs, and one might reasonably speculate that efficient DNA repair is needed in cells of long life span with no or limited regeneration from precursor.
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