Sentence examples for differentiated day from inspiring English sources

At the end of day 6, DMII medium was changed to basal media (BMII), and the cells were maintained in BMII (replaced with fresh basal medium every 2 days) until fully differentiated (day 12).

We identified that 100 ng/mL BMP4 alone resulted, after 72 hours, in a doubling of the number of terminally differentiated day 30/31 chorionic girdle trophoblast cells most likely by promoting terminal differentiation because proliferation was not induced with treatment.

Representative chromatograms of GSLs in (a) undifferentiated (Day 5), (b) confluent (Day 7), (c) partially differentiated (Day 14), (d) differentiated (Day 21), and (e) post-differentiated (Day 24) Caco-2 cells.

In this study, cell surface GSLs were monitored in vitro at five different time points: undifferentiated (Day 5), confluent (Day 7), partially differentiated (Day 14), differentiated (Day 21), and post-differentiated (Day 24).

Venn diagram showing the number of genes with LSD1 peaks at promoter in differentiated (day 7) (a) 3T3-L1 Ctrl) and 3T3-L1 LSD1 3T3-L1 LSD1-10T1/2(Ctrl) and C3H-10T1/2(LSD1) cells.

Relative transcript levels of the indicated markers in differentiated (day 7) (c) C3H-10T1/2 C3H-10T1/2-L1 cells treated with vehicle or CL316,243 and transfected with unrelated control siRNA (siCtrl) or siRNA directed against LSD1 (siLSD1).

The level of promoter methylation observed in cells of the smaller SAT from 10 week-old WT mice was greater than that observed in the larger, more differentiated SAT of db/db mice or in the SAT of 20 week-old WT and DIO mice.

We next analysed the expression of Tnfsf11, Tnfrsf11b and Il6 in terminally differentiated osteoblasts (day 30 of differentiation) and in primary chondrocytes (day 20 of differentiation).

3T3-L1 cells were differentiated 1 day after they became confluent (designated as day 0) using the DMI protocol (1 μM dexamethasone, 0.5 mM 3-isobutylmethylxanthine, and 1 μg/mL insulin in DMEM with 10% FBS) as detailed in Supplemental Material, Figure S1 (http://dx.doi.org/10.1289/ehp.1205731) and described previously (Hou et al. 2012).

Contracting monolayers of differentiated cells (day 16) were maintained for 5 days in each test condition before measurement.

All experiments were performed in fully differentiated adipocytes (day 8).