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To demonstrate that resistance of NK cell mediated cytotoxicity by increased differentiation of stem cells is not restricted to only certain types of cells, we used monocytes and their differentiated counterpart dendritic cells to determine sensitivity to NK cell mediated lysis.
Neuroblastoma and its benign differentiated counterpart, ganglioneuroma, are paediatric neuroblastic tumours arising in the sympathetic nervous system.
We next compared LCSC response with erlotinib and EGFR activation level with that of their in vitro differentiated counterpart.
We found that erlotinib sensitivity of EGFR-WT LCSCs was even higher when compared with their differentiated counterpart, in line with their higher level of receptor activation.
We also applied GSEA for the regulatory network changes associated with development using comparisons between a competent skin against its differentiated counterpart — E7 feather vs. E9 feather; E9 scale vs. E11 scale.
As shown in Fig 3C (left and right panels) in both conditions the protein levels of Pin1 and N1-ICD were almost two times higher in the stem cell fraction as compared to those in their differentiated counterpart.
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In order to fully characterize the differentiated GBM cells subjected to this dedifferentiation protocol, we first checked by RT-qPCR the expression of several stem and differentiation markers in NS-derived cells and their differentiated counterparts.
Moreover, as DEK may be present at higher levels in immature cells than in differentiated counterparts, it could also aid in gauging the differentiation potential of tumor cells.
The aims of this study are to demonstrate the increased lysis of stem cells but not their differentiated counterparts by the NK cells and to determine whether disturbance in cell differentiation is a cause for increased sensitivity to NK cell mediated cytotoxicity.
Cancer stem cells exhibit distinctive cellular metabolism compared with the more differentiated counterparts or normal cells.
TF and IMR-90 served as differentiated counterparts for ES and iPS cell lines, respectively.
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