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The three neurofilament genes (NF-L, NF-M, NF-H) were differentially expressed in the undifferentiated and differentiated conditions, possibly as a consequence of being independent transcription units and hence were differentially regulated [26].
Fig. 2 Summary of results of E449-catalyzed hydrolyses of 1 under differentiated conditions.
We assayed MSChUCB1 for the presence/absence of CD133 expression under control and differentiated conditions (Fig. 3C).
When vole TS-like cells are maintained in differentiated conditions for 12 to 14 days, they lose Tsix expression completely, most likely due to repression of the Tsix promoter on the active X chromosome (Fig. 5A, lane 5).
Second, three differentiated conditions were applied in order to distinguish the two major incentives to free-ride, which are 'fear of losing money' and 'greed for earning more money than others' [19].
By comparing the expression data of IKAP-DR cells from those of control cells in differentiated conditions, we identified 180 genes whose expression was either elevated (99) or reduced (81) in the IKAP-DR cells compared to control cells (Table S4).
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Thus, we estimated that the chondrocytes in our study were maintained in a differentiated condition.
The term "epigenetic landscape" was coined in 1939 by CH Waddington, before DNA was recognized as the molecule of inheritance, to describe the mechanisms of transition of the cells from the totipotent to the differentiated condition.
Category A included the IKBKAP gene itself, which was reduced in non-differentiated conditions because of IKBKAP shRNA expression, and further reduced upon differentiation (Figure 1).
To investigate possible roles of IKAP during neuronal differentiation, we compared gene expression changes in IKAP-DR and control cells in differentiated versus non-differentiated conditions.
They further showed the effectiveness of regulation of transgene expression by cell type dependent miRNA expression using hESC in pre- and post-differentiated conditions [18].
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