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Significant current attention is paid to improving chondrogenic differentiation capacity; unfortunately, the potential chondrogenic hypertrophy of differentiated cells is largely overlooked.
Reviewer Reply: "newly differentiated cells" is what was written, but "newly differentiating" and "newly differentiated" are hard to really to distinguish regardless.
These tumours retain hallmarks of the normal epithelial terminal differentiation programme; however, proliferation is increased, the proportion of differentiated cells is decreased, and the spatial organisation of the cell layers is disrupted [1].
A more likely explanation for the performance gap between predictive models for embryonic stem versus differentiated cells is the increasing heterogeneity in regulatory mechanisms following differentiation.
This division of labour preserving the replicative potential (stem cell) and carrying out the specific functions of the organ (differentiated cells)—is mimicked in tumours, but in a less-organized fashion.
Understanding specific cargo distribution in differentiated cells is a major challenge.
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Differentiation induces Olig2 expression and differentiated cells were selected based on labeling with Olig2 antibodies.
Differentiated cells were co-stained with adiponection to verify differentiation.
Differentiated cells were co-stained with adiponectin to verify differentiation.
The differentiated cells were used after 9 days of initiating the differentiation.
The differentiated cells were used after 4 or 6 days of initiating the differentiation.
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