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Secondary NS were differentiated by removal of bFGF for 72 h.
This dataset was obtained from RNA samples collected at D0 (Day 0), D1, D2, D3, D5, D7, and D9 from primary OPCs that were differentiated by removal of the mitogen platelet derived growth factor (PDGF-AA) and the addition of thyroid hormone (T3) [25].
After two passages, cells at 80% confluence were differentiated by removal of βFGF in a culture medium containing 50% of DMEM-F12 with modified N2 supplement and 50% Neurobasal with B27 supplement (Invitrogen Life Technologies, Cergy Pontoise, France).
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We therefore induced apoptosis in differentiated KIM-2 cultures by removal of the lactogenic hormones prolactin, dexamethasone and insulin.
Moreover, although submerged cells that have not undergone organotypic differentiation can be exposed directly to complex aerosols such as CS, 9– 12 the nonspecific stress and damage caused by removal of the culture medium and the lack of differentiated features that protect against airborne toxicants (eg, mucociliary or squamous differentiation) cannot be excluded.
Finally, kidney was chosen as a highly differentiated tissue that does not appear to have substantial proliferative capacity, and does not appear to be affected by removal of E2f4.
In this section, we examine the behavior of a cell initially in a differentiated state to removal of differentiation stimulus, and investigate a role for stochasticity in triggering transitions from a differentiated state to the pluripotent state.
Typically these machines are differentiated by d.p.i.
There are two Andersons, differentiated by a middle initial.
First it segments the picture by tracing regions that are differentiated by color.
All species are best differentiated by their vocalizations.
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