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When C2C12 cultures reached 70 90 % confluency they were differentiated by changing the media to differentiation medium consisting of DMEM supplemented with 2 % horse serum, 200 U/ml penicillin and 200 µg/ml streptomycin.
When at confluence, myoblasts were differentiated by changing SGM with SDM.
Two days later, NSPCs were differentiated by changing the media to NBA containing 1X PSQ, 1X B27, and 1% FBS.
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For the adhesion assays, the Caco-2 cells (passages 42 65) were seeded at 1 × 10 cells/mL (preconfluence) in 24-well tissue culture plates (Corning, Inc., Corning, NY, USA) and fully differentiated for 16 d (postconfluence) by changing the culture medium every 2 d.
hESCs were differentiated after plating in mTeSR-1 by changing the medium to DMEM/F12 with 10% Bovine Growth Serum (Hyclone), and culturing for an additional 6-8 days.
Integrins enable cells to sample the topology and mechanochemical properties of their pericellular environment and respond by changing their position and differentiated state [ 1,2].
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The ability of MSCs to differentiate into osteoblast and chondrocytes were identified in vitro by changing their morphology as labeled by arrows in Figure 3b, c and by their staining with special staining as Alzarin red and Alcian blue for differentiated cells respectively as shown.
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