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For differentiation experiments (n = 3, df = 2); cells were differentiated as described [74] in the presence or absence of cleaved (600U/mg) or monomeric (50 U/mg) TRAP (10−9 M–10−12 M).
For differentiation experiments (n = 3, df = 2); hMSC (passage 18) were differentiated as described [72] in the presence or absence of cleaved or monomeric TRAP (10−9 M).
Monocyte derived immature dendritic cells (imDC) were differentiated as described before39.
C17.2 cells were seeded on poly-d-lysin/laminin-coated coverslips and exposed and differentiated as described for the immunocytochemistry protocol.
The Simpson-Golabi-Behmel Syndrome (SGBS) human pre-adipocyte cell strain was provided by Dr. Martin Wabitsch (Ulm University, Germany) and cultured and differentiated as described previously (Wabitsch et al., 2001).
Human preadipocytes were differentiated as described with increasing concentrations of BPA for 14 days, and protein levels of the adipogenic marker aP2 were assessed by western blotting (a) and densitometry (b) analyses.
Preadipocytes were cultured and differentiated as described before [23].
These cells were then differentiated as described above.
Pre-adipocytes were isolated and differentiated as described [25].
Human adipocytes were cultured and differentiated as described elsewhere [21].
PC12 cells were cultured and differentiated as described elsewhere [23].
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