Sentence examples for differentiated and treated from inspiring English sources
For e h, iWAT precursors were differentiated and treated during 24 h with GW9508 (100 μM, blue bars; n=5) or nor treated (n=3).
When these macrophages were fully differentiated and treated with the TLR2 ligands: LTA, R-FSL1, S-FSL1, Pam2CSK4, or Pam3CSK4, once again there were ligand-specific activation kinetics of the p38, SAPK/JNK, and NF-κB pathways, which precisely matched those observed using the immortalized Raw 264.7 cells (Fig. 2A C).
Cells were plated at 1 × 10 cells/well in 96 well plates, cultured, differentiated and treated as described above.
3T3-L1 cells were differentiated and treated with RM (0-300 ug/ml) and expression of FAS, ACS1, FABP4 and FATP1 evaluated by RT-PCR as described in Materials and Methods.
To test the involvement of TRPM5 in ATP-induced MUC5AC secretion, wild-type and stable TRPM5-depleted N2 cells were differentiated and treated with ATP for 30 min. Knockdown of TRPM5 significantly reduced ATP-induced secretion of MUC5AC.
To identify genes affected early by the HDIs, we differentiated and treated MC3T3-E1 cells with three HDIs or vehicle for 18 hours and analyzed gene expression profiles with Affymetrix microarray gene chips.
Primary myoblasts were differentiated into myotubes and treated with agrin.
3T3-L1 pre-adipocytes were differentiated into adipocytes and treated with RM (0-300 μg/ml).
Human monocytes were differentiated into macrophages and treated with M1, M2, and M8 in the presence or absence of LPS.
Total RNA was isolated from proliferating myoblasts and differentiated cell cultures and treated with DNase I using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
DM1 fibroblast cells with inducible MyoD were differentiated to myoblasts and treated with chromomycin A3 and Ro-31-8220 at concentrations of 40 nm and 10 μm respectively, for 48 h, and the resulting splicing profiles analysed.
