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Furthermore, clonally expanded CD51+ cells were induced to differentiate under lineage-specific differentiation conditions, these cells showed robust differentiation potential, including adipogenic, osteogenic, and chondrogenic ability.
The precursors of the T lymphocytes migrate from the marrow to the thymus, where they differentiate under the influence of a hormonelike substance.
HMF-exposed and GMF primary NSs from P2 mice (day 7) were collected and induced to differentiate under the GMF condition for 5 days.
Therefore, lack of LRRC25 instead of off-target effect of Cas9 in the knockout cells contributed to the impaired ability to differentiate under ATRA treatment.
Regulatory T lymphocytes mediate homeostasis of the immune system and differentiate under the control of the transcription factor FoxP3 in the fetal thymus.
In addition, we restored LRRC25 expression in 5F8 (KO) cells (Fig. 5J) and tested whether restoration of LRRC25 could rescue its ability to differentiate under ATRA treatment, using NBT reduction assay which is a standard test to evaluate granulocytic differentiation.
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Under conditions in which differentiation would be induced in NSCs, GSCs showed an intrinsic potential to maintain their undifferentiated state or to resist differentiation and even tended to retro-differentiate under certain circumstances.
Analyzing neuronal differentiation we demonstrated feeder-freed stem cells to exhibit differentiation potentials comparable to embryonic stem cells differentiated under standard conditions.
To test the differentiation potential of these cells, Day 12 hSKPs were differentiated under different conditions for 2 4 weeks.
We demonstrated that incubation of TCR-transduced hematopoietic progenitors with the combination of coculture systems gave rise to CD7+TCR+CD3+CD1a− T cell precursors that rapidly proliferated and differentiated under the culture condition to induce mature T cell differentiation.
Both cells successfully differentiated under osteogenic, adipogenic, and chondrogenic microenvironments; PDLSCs displayed a more effective differentiation potential in all of the incubation conditions compared to GMSCs (P < 0.01).
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