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Thus, teleost-specific duplicated genes seem to be on a path to functional differentiation, namely, 3R occurred neither too recently nor too early to differentiate the function of paralogs.
Our hypothesis was developed by using a strategy of homocysteine (hCys) for Cys substitution in the Cys-Cys redox dyad of DmTR to differentiate the function of each Cys residue.
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The specific down-regulation of the target gene transcript level within at least 24 hours post-injection was clearly observed (Figure 5C), which makes it possible to differentiate the functions of the two trehalase genes.
In order to differentiate the functions of foxi3a and foxi3b more precisely, we sought to create a genetic background which contains no endogenous foxi3a or foxi3b expression by misexpressing X-Su(H /Ank mRNA or bmp7 MO.
The role of the motif 8 is not annotated in the protein family (Pfam) database (http://pfam.sanger.ac.uk/), but its loss may be sufficient to differentiate the functions of the Vrs1 and HvHox2 gene products.
Most authors have assumed this pivotal role to depend on GSK3β (Peineau et al., 2008), despite using general GSK3 inhibitors which cannot differentiate the functions of the GSK3 isoforms (Cohen and Goedert, 2004; Georgievska et al., 2013).
Thus, the molecular mechanisms differentiating the function of MST1 and MST2, as well as the function of MST2 during muscle atrophy, need to be elucidated in future studies.
Since the Japanese government launched the health care reform in 2004 to reduce medical costs by differentiating the functions of medical institutions [ 13], an investigation of the quality of primary care, especially in terms of patient-physician relationship, is merited.
These studies have derived MSC-like cells that have shown biological potencies similar to adult MSC, but have not addressed the need to differentiate and assess the function of stromal fibroblasts.
Efforts focused at differentiating or attenuating the function of MDSCs have been promising, leading to improved immune response against the tumor and a better prognosis for the patient [2].
One included tool was able to differentiate between the functions of the general nurse and the advanced practice nurse by the level of activities undertaken within the five domains described.
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