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Cells induced to differentiate, as well as undifferentiated cells were assessed for gene and protein expression using quantitative polymerase chain reaction and immunofluorescent staining, respectively, for osteogenic and myogenic markers.
The SR-BI/CLA-1 knockdown cells were able to grow and to differentiate as well as untransfected Caco-2/TC7 cells (data not shown).
Activated satellite cells differentiate to myoblasts, which proliferate and differentiate as well.
Moreover, ex vivo studies showed that dtd mice osteoclasts are able to differentiate as well as wild-type cells.
Chemical biologists therefore have an opportunity to make unique contributions to our understanding of how pluripotent cells self-renew and differentiate, as well as our ability to control these processes for therapeutic purposes.
Moreover, T cells exhibit plasticity enabling some populations to change their functional properties depending on the environmental cues they receive both as they differentiate, as well as when they function in vivo (4).
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We found that satellite-derived cells from mdx mice showing poor differentiation after two days differentiation, recovered and differentiated as well as controls after three further days in differentiation conditions.
The identity of these ectopic cells is not clear, and often neighboring layer cells mis-differentiate as well.
Many individual mdx mice yielded satellite cells capable of differentiating as well as age-matched controls.
Cells from whole cord differentiated as well as or better than those isolated from individual cord regions and therefore have potential as a useful source for obtaining promising cell populations for further study.
Trichoptera larvae display a wide-range of ecological diversity by exploiting a variety of habitat types and occupying various trophic levels by temporally differentiating as well as employing different life strategies [ 2].
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