Also, proper parameterization of long-range as Coulomb and non-central many-body forces as charge transfer interactions and covalency effect, short-range as overlap repulsion extended up to the second-neighbor ions, van der Waals interactions and zero point energy effects are differentially needed.
Of course, consolidation in memory certainly seems to be an instance of an active memory operation, but the present paradigm did not specifically implicate that particular function (i.e., consolidation was not differentially needed to do the memory task).
A practical method for the synthesis of cis-1,2-cyclopentanedithiols and cis-3,4-pyrrolidinedithiols with differentially protected sulfurs, needed for the design of new metal-chelating ligands, has been developed.
Furthermore, polyploidy differentially regulates genes needed for MEG maturation and platelet production 22.
To generate the list of differentially expressed genes needed for the analysis with GeneCodis, we used the topTable function from the bioconductor Limma package in R to obtain a ranked list of probes with the most evidence of differential expression between the knockout and wild-type samples.
To determine if α- and μ2-adaptin contribute differentially to endocytosis, we needed to identify specific cargo.
For many hypothesis-driven investigations or validation of genome-wide methylation studies, analysis of specific gene promoters, CpG islands or differentially methylated regions is needed.
However, as the structural complexity of biological systems increases and connectivity of multiple parts becomes non-linear, new molecular strategies are needed to differentially utilize the genome in distinct cell lines of eukaryotic organisms, to coordinate development and cellular identity and to structure and stabilize genome environment interactions.
Good performance of screening group differentially expression genes needs a high correlation among the same replicates.
Appropriate statistical tools, including normalization, clustering, and identification of differentially expressed genes need to be applied in any microarray experiment.
When testing 20,000 genes with a per-gene alpha value of 0.05 the number of samples needed to detect differentially expressed genes by t-tests with a fold change of 1.2 is 112, whereas only eight samples are needed to statistically have the power to detect a 2-fold difference (http://bioinformatics.mdanderson.org/MicroarraySampleSize/MicroarraySampleSize.aspx).aspx
