Thus, the objective of this study was to investigate whether activation of the ERK1/2 pathway was involved in the differentiation of BMSCs cultured on differentially modified surfaces into an osteogenic lineage.
The GSH/GSSG ratio was differentially modified by the actives tested, providing important insights into their specific mechanism of action.
Using human-engineered and differentially modified microtubules we find that glutamylation is the main regulator of the hereditary spastic paraplegia microtubule severing enzyme spastin.
Mass spectrometry of differentially modified cysteines, and Edman degradation analyses identified Cys 22 Cys 157 and Cys 191 Cys 220 as DDT-sensitive.
Total lignin content, composition in syringyl and guaiacyl units, and absolute proportions of condensed and non-condensed substructures occurring in the cell walls, were differentially modified according to the repressed gene.
For instance, Figure 2 demonstrates sequences of six differentially modified peptides corresponding to the N-terminus of H2AFZ.
To determine the oxidation state of cysteines they were differentially modified using a method described by Creighton [22].
Modified amino acids are recognized by chromatin-binding factors that distinguish between differentially modified histones [5], [6], [7] and are involved in the organization of chromatin [8], [9].
Interestingly, we sequenced two differentially modified N-terminal peptides from the earlier uncharacterized variant of histone H4, encoded by the HIST2H4B gene.
The modifications of ICP0 required during the inhibition of IRF3-mediated ISG induction are of interest, especially in light of data showing that ICP0 is differentially modified in the nucleus as compared to the cytoplasm [29].
Besides the alternative removal of the methionine, the N-terminal peptides of H2AFZ were differentially modified and contained between three to five different modifications by methylation, acetylation/trimethylation, phosphorylation and ubiquitination (Figure 2).
