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By differentially labelling the cells in these domains with fluorescent proteins, it has been shown that filopodia, as well as providing the mechanism for knitting epithelial edges together, can also 'sense' matching opposing cells and sort these epithelial domains.
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Differentially labeled heavy- and light-labeled peptides co-elute from the LC.
BrdU-labeled DNA from each fraction is immunoprecipitated (BrdU IP), amplified, differentially labeled and co-hybridized to a whole-genome comparative genomic hybridization microarray (or sequenced).
Simultaneous detection of two kinds of bacterial is achieved by the design of differentially labeled TaqMan-MGB fluorescent probes.
RNA was isolated from treated and untreated cells, differentially labeled with fluorescent dyes, and then hybridized to the array.
For the competitive sprouting assay, we mixed differentially labeled ECs with eGFP or tdTomato at a 1 1 ratio and then analyzed at 8 h after the fibroblast seeding.
Before applying Chip-SIP to natural communities, we sought to test its sensitivity and ability to discriminate a mixture of differentially labeled bacterial taxa in controlled mixtures.
We developed sparse coculture for connectivity assays where SHANK2 and control neurons were differentially labeled and sparsely seeded together on a lawn of unlabeled control neurons.
We also use the pUltra plasmids to produce mixtures of differentially labeled pathogenic Escherichia, Shigella and Salmonella species which we test during infection of mammalian cells.
Glucose first-pass uptake by the splanchnic tissues was calculated from the rate of appearance of differentially labeled glucose tracer in plasma.
The strategy extends the size limit for structure determination by NMR spectroscopy to 42 kDa for monomeric proteins and to 65 kDa for differentially labeled multimeric proteins without the need for deuteration or selective labeling.
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