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This differential splicing pattern was detected in 13 of 18 patients (72%).
RT-PCR of AdCa samples confirmed the differential splicing pattern measured using the exon array in four cases.
With regard to these probe sets, we found that three genes exhibited a more pronounced differential splicing pattern in AdCa (GTPBP10, DUOX1, and KIAA1217), whereas 13 genes had a more pronounced pattern in SCC (e.g. CD44, FN1).
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Accordingly, we next investigated the splicing status of all genes and whether genes show differential splicing patterns between normal and diseased tissues.
Despite high structural similarities of the respective splice sites, both in vitro and in vivo experiments have demonstrated differential splicing patterns of the 3 NRXN1/2/3α in response to activation.
We used the GeneChip® Human Transcriptome Array 2.0 (HTA 2.0) for analyzing differential gene expression studies to analyze differential splicing patterns in NT and iAs-T cells.
Several genes have been described to exhibit differential splicing patterns for different tissues (E.g. PDE1C [ 1]; IRF-3 [ 2]) that result either in alternative proteins or affect the regulation of the respective gene product [ 3].
Sequencing cDNAs encoding Csn1s1, Csn2 and Wap in both mouse species revealed differential splicing patterns and SNPs in coding sequences, of which some were responsible for amino acid substitutions.
Note that the advanced tumor sample in Lane 13, the most predictive for our differential splice patterns, does not display alternative splicing of CD44.
Concomitantly, such a duplication will allow differential splice patterns (intron retentions) as a temporary or permanent alternative to the establishment of a constitutive intron if the duplication size is a multiple of three.
Most notably, the pattern of differential splicing ratios is similar for all analyzed genes.
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