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Warner, J.A. et al. Differential release of mediators from human basophils: differences in arachidonic acid metabolism following activation by unrelated stimuli.
A polymer drug conjugate nanocarrier is developed for the concurrent delivery of a cocktail of therapeutic agents at predefined ratios and with differential release kinetics.
Three types of early release studies were used: single 60 s study (single cup), 10 s followed by 50 s (two cups) and 10, 20, 30 … 60 s multiple changeover differential release (six cups).
This differential release is associated with differential expression of activity-dependent genes, such as egr1 (avian zenk), which in mammalian brain are modulated by dopamine receptors.
Differential release of MC mediators further supports the existence of separate granule populations.
The finding is in agreement with previous reports indicating differential release of MC mediators.
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In vitro drug release studies were carried out to investigate a time-differential release of the drugs.
The dual drug-encapsulated nanoliposomes showed a time-differential release of ERL and DOX, implying proper sequential releases for their synergism.
The in vitro time-differential release of DOX and ERL from the dual drug-encapsulated nanoliposomes was investigated, and the results are shown in Fig. 8.
ERL and DOX co-encapsulated in the nanoliposomes showed a time-differential release, indicating much faster release of ERL than that of DOX from the liposomes.
These results suggest that during the initial period of release test, much more amounts of ERL were released from the liposomes than those of DOX and there was a time-differential release between ERL and DOX.
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