Gene expression analysis was conducted using SYBR Green-based qPCR and individual primer assays for differential miRNA expression analysis and performed on the CFX96 Real-Time instrument (BioRad, USA).
Some published tools have the function performing differential miRNA expression analysis between samples [ 3, 4, 7, 24].
The first approach to identify molecular markers capable of predicting tumor response to nCRT was to perform differential miRNA expression analysis using the 27 samples from the training set.
By combining target gene prediction for differential miRNAs, joint analysis of mRNA expression profiles, and BLAST analysis, the critical role of let-7b in regulating the GHR expression was identified.
In next step, all normalized experimental data were imported in GeneSpring GX 12.5 software for the differential miRNA expression, fold change analysis, and cluster analysis.
To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines.
Luo X, Gu J, Zhu R, Feng M, Zhu X, Li Y, et al. Integrative analysis of differential miRNA and functional study of miR-21 by seed-targeting inhibition in multiple myeloma cells in response to berberine.
An analysis of differential miRNA expression revealed that let-7c was significantly downregulated in the sputum of currently smoking patients with chronic obstructive pulmonary disease [ 29].
Future directions involving miRNAs in the Black flying fox include analysis of differential miRNA expression, correlating miRNA expression with that of putative gene targets, and experimental validation of targets for individual miRNA candidates.
