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ns: log2-based differential expression ratio of the gene not significantly differentially expressed.
We then selected a non-redundant list of 758 LUAT expressed in either DN or DP cell stages and compared their differential expression ratio along with the expression ratio of the associated coding-genes.
For each human gene, we obtained its Differential Expression Ratio (DER) from the study by Chen et al. [16], [17].
We obtained the differential expression ratio (DER) of human genes from the study by Chen et al. [16], [17].
Based on the differential expression ratio inferred from all human microarray data in NCBI GEO and the list of disease genes curated in public repositories, we systemically analyzed the general relation of AP repertoire with expression diversity and disease susceptibility.
A list of putative anchor genes were selected as those displaying; a) the most significant differential expression ratio (>2 x median expression fold change), b) high raw signal intensity (>100 RFU) above background and c) low median signal intensity in all other subcompartments examined (Figure 1).
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The numbers are log2-based differential expression ratios from the microarray analysis.
To calculate differential expression ratios, all identified spectra from a protein were used to obtain an average protein ratio relative to the control label (i.e. fold change).
To ascertain the relationship between the transcript expression and protein abundance, we compared the differential expression ratios observed in the two datasets.
Differential expression ratios for proteins were obtained from Protein Pilot™ which calculates protein ratios using only ratios from the spectra that are distinct to each protein, excluding the shared peptides of protein isoforms.
The differential expression ratios were higher than 2 fold for most proteins and western analyses were performed by using selected antibodies to verify the 2-D PAGE proteomic data for few proteins (Fig. S2).
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