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From this library, 222 plasmid DNAs were screened by differential dot hybridization, and 14 clones hybridized strongly to rye DNA, but did not hybridize to wheat DNA.
Each PCR clone was subjected to differential dot blotting to identify those that hybridized preferentially to the tester XaFL07-1 DNA.
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For differential system (dot {x}=h t,x)), where (h t,x)) is discontinuous in x.
The dynamics of the controlled epidemic are given by the non-linear differential equations (dots denote time derivatives, i.e. : (1) where s(t) + i(t) + r(t) + d(t) = 1, s(t), i(t), r(t), d(t) ≥ 0, and the control, u(t), takes values in [0, b].
Seventy potential BAN specific sequences were obtained after SSH of the BAN genome, with a pool of closely related strain genomes and subsequent differential screening by dot blot hybridisation.
The construction of the libraries, clone sequencing, vector and adaptor trimming and differential screening using dot blot hybridization were outsourced to Rx. Bioscience Ldt (Maryland, USA).
For the differential screening by dot blotting, 1000 clones per library were randomly transferred on two nylon membranes and hybridized with C and FA cDNA probes, respectively.
d Red dots represent differential travel times between direct S and reflected S waves for each source (Fig. 7a).
Fiore et al. [8] have studied the effects of intradot relaxation on the K-factor and differential gain of quantum-dot lasers.
It seems unlikely that these differences in slope were due to differential salience of individual dots, or shape-specific factors that would modify information persistence.
Red dots represent significant and blue dots non-significant, differential expression of up- and down- regulated genes.
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