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We used RT-PCR differential display in BTH-treated cucumber plants to detect SAR associated mRNAs.
High-sensitivity molecular analysis methods such as LMM and differential display, in combination with molecular imaging of 'vehicle' cells, genes and proteins of interest, present a valuable tool to obtain novel insights into compartment-dependent synovial pathways.
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In A549 cell model, 164 dynamic changed spots (%volume have two times differential displayed in any two time points in CBB image or PH-I image or both) were detected and only 43 among them have the same change trend in both expression and total amount level.
Scotin was identified by a differential display approach in search for new genes involved in p53-dependent apoptosis induced by irradiation of normal and p53 nullizygote mice [17].
IκBζ was first identified by a differential display analysis in a variety of tissues upon i.p. injection of LPS in wildtype mice [ 61].
Comparison of qualitative expression assessment of vascular markers by human breast cancer cells MCF-7 (poorly aggressive), MDA-MB-231 (MB-231; highly aggressive) and HUVECs, as shown in the raw data from the ribonuclease protection assay and differential display analysis in Figure 1.
In conclusion, affordable, available and accessible clinical data collected in a standardized manner and analyzed according to the rules of differential display result in an accurate description of the phenotype of patients with a complex disease, e.g. symptomatic arteriosclerosis.
Fragments of all CCT subunits except the α subunit, which had already been obtained through a differential display analysis in the previous study [25], were obtained using degenerate PCR and RACE.
We performed a cDNA chip-based differential display analysis, in order to identify overexpressed genes of bladder carcinoma, as compared to normal bladder mucosa.
The latter consists of various data mining methods from public database including digital differential display (DDD) in non-normalized cancer genome anatomy project (CGAP) libraries and digital Northern-blot in National Cancer Institute (NCI) SAGEMap [ 8, 9].
Dido1, the smallest of these, was identified by differential display PCR in murine bone marrow cells undergoing apoptosis and its ectopic overexpression was shown to induce caspase-dependent cell death.
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