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Differential Display was chosen to identify differentially-expressed genes in SHE cells and to explore the entire genome.
We used a differential display strategy to identify candidate genes differentially expressed in the hypothalamic region of the exposed tadpoles.
Differential display RT-PCR was employed to identify differentially expressed genes affected by progesterone and EGF.
We then employed differential display RT-PCR [ 9] to identify differentially expressed genes affected by progestin and EGF.
Genes differentially expressed in normal versus tumor tissues were identified using a computer-based differential display strategy.
The differential display method was performed as described previously using a Fluorescent Differential Display Kit and LA Taq polymerase with a combined total of 216 primer sets (Takara) [21], [22].
Subtractive cDNA hybridization [18] and fluorescent differential display [29], [30] are also effective at identifying imprinted genes.
AC performed the Differential display analysis.
RNA was isolated and analyzed using differential display fingerprinting.
Overall, ten differential sequences were obtained through differential display analysis.
The mRNA differential display was performed using the differential display kit from Eurogentec Belgiumm) and following the instructions of the supplier.
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