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Upregulation of genes encoding different types of nutrient uptake systems further emphasized the differences in nutritional requirements of the two bacteria in the same insect host.
0.3 g of Urea and Ammonium Sulphate were added separately to the samples to provide different types of nutrient for the Trichoderma pseudokoningii growth.
To create a synthetic model of obligate mutualism, we constructed two different types of nutrient auxotrophs of E. coli by genetic recombination (Fig. 1A): an isoleucine (Ile) auxotroph, designated I–, labeled with a red-fluorescent protein (dsred.T3), and a leucine (Leu) auxotroph, L–, labeled with a green-fluorescent protein (gfpuv5) [22] (see Methods).
Induction of different types of nutrient uptake systems in Photorhabdus and Xenorhabdus further emphasized the possible differences in nutritional requirements of the two bacteria in the insect host.
We suggest that these different functions of the mounds result in different types of nutrient enrichment, which will play an important role in the effects of the mounds on the surrounding vegetation.
In this study we made an investigation into the cell growth and lipid accumulation along with the transition from heterotrophy to three nutrient-deficiency cultivations, and compared the regular patterns of physiological parameters and transcriptional data between different types of nutrient starvation.
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These variables may include concentrations of different types of particles, nutrients, reaction products and residual products.
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