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The studies were first divided into two main groups according to the laboratory method: IHC or molecular biology, including the different techniques of PCR.
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Frequency differences may arise due to the use of different techniques (real-time PCR versus IHC) on separate tumour cohorts (fresh frozen versus FFPE) and accomplishing different scoring systems.
Samples were analyzed by two different techniques: detection of influenza viruses by using a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) in an ABI Prism 7300 thermocycler (Applied Biosystems) [ 12] and virus isolation.
Real-time PCR assays may be helpful to detect integrated HPV forms but further studies on greater number of specimens comparing different techniques for detection of integrated HPV-16 need to be conducted.
Due to the lack of available data, first we verified the expression and activity of CFTR in human macrophages by different techniques; i.e. RT-PCR, immunofluorescence, patch clamp recording.
In our study two different techniques (i.e., RT-PCR and IHC) were used to evaluate MGB-2 expression.
Further studies, using different techniques such as quantitative RT-PCR or protein extraction on frozen samples, will be needed to accurately assess the expression of CCL20 and CCL21 in primary breast cancer.
We have analyzed subtypes of HIV-1 in a group of HIV-1 positive IDUs using two different techniques, i.e., subtype-specific PCR (Fig. 1) and HMA (Fig. 2).
There are a wide range of commercially available HPV detection assays which are based on different techniques such as target amplification (mainly PCR), signal amplification, and probe amplification [ 26].
The detection sensitivity of KDCC was also evaluated with two different techniques, i.e. a multiplex-PCR and a digital DNA hybridization by digital DNA chip analyzer (DDCA), using natural plankton assemblages.
Different labs have mastery of different techniques.
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