However, chromosomal abnormalities during the meiosis showed significant differences at different pollen sterility loci in autotetraploid rice hybrids.
We focused on the interactive effects of different pollen sterility loci and the relationship between single pollen sterility locus and multiple pollen sterility loci, especially on the pervasive interactions at Sa and Sb loci in autotetraploid rice hybrids.
Our results indicated that hybrids with the interactions of different pollen sterility loci exhibited significant differences in the pollen fertility compared to the parent Taichung65-4×.
It is worth mentioning that significant differences were detected in the number of DEG in the hybrids with the interactions of different pollen sterility loci (without neutral genes), such as single F1 pollen sterility locus interaction (Sa, Sb, and Sc), double loci interaction (SaSb), and triple loci interaction (SaSbSc).
The profiling of the DNA methylation under two different phenotypes (sterility and fertility) revealed that hypermethylation was observed in PA64S (sterility), and 1258 differentially methylated regions (DMRs) were found between PA64S (sterility) and PA64S (fertility).
In the present study, we used near-isogenic autotetraploid rice lines to develop autotetraploid hybrids with different pollen sterility loci interactions, including with Sa, Sb, Sc, SaSb and SaSbSc pollen sterility loci interactions.
Chromosome behavior analysis during meiosis was also used to evaluate the interaction effects of different pollen sterility loci.
There might be various interaction networks at different pollen sterility loci in hybrids, which required further studies.
Frequency of abnormal chromosome behaviors in autotetraploid rice hybrids harboring the interactions of different pollen sterility loci.
To explore the possible interaction effect associated with different pollen sterility loci in autotetraploid rice hybrids, gene expression profiling analysis of different autotetraploid rice hybrids was conducted.
