To achieve the aforementioned properties, these methods incorporate different steps such as generative part models [59, 73], discriminative codebook learning [68, 74], sparse coding [60, 62, 66], and/or spatial pooling [62].
Different steps such as DNA extraction, sample preparation, amplification and post-PCR were performed in strictly separated rooms.
For detailed discussions on the performance for different steps such as variant calling please see the publications for the respective tools.
Model development is a key task in systems biology, and involves different steps, such as model calibration, experimental design and model refinement which usually take place in an iterative way (see reviews in [ 1- 5]).
Modern manufacturing of printed wiring boards (PWBs) involves extensive use of various hazardous chemicals in different manufacturing steps such as board preparation, circuit design transfer, etching and plating processes.
We tested antibiotics targeting different translation steps, such as the aminoglycosides: G418, paromomycin, ribostamycin, tobramycin, neomycin and kanamycin [19], as well as DNA damaging agents like bleomycin and phleomycin [20].
Learner reaction to an educational experience can be assessed through surveys that use questions appropriate to teaching different EBP steps, such as: Did the instructor's teaching style enhance your enthusiasm for asking questions during ward rounds?
The DBToolkit from the Compomics framework (http://dbtoolkit.googlecode.com) provides a GUI to build sequence databases, after performing different processing steps such as protease digestion, decoy and sequence pattern filtering.
Noller [ 19] has proposed that the ribosome evolved from "smaller functional units capable of carrying out the different translational steps such as peptidyl transfer, decoding, and so on", similar to the view of Penny and co-workers, who have stated, "It is possible that the several active sites of modern ribosomes evolved as separate ribozymes" [ 4].
For instance, if Y2H has been the most popular strategy so far to construct VH interactomes, technological variations of this generic approach are very important at different essential steps, such as the reporter genes, yeast strains, plasmid copy number, fusion proteins, stringency conditions and libraries, that have an obvious impact on the outcome of the experiment [ 53].
According to Bass, specific interventions are based on the notion that progression of NAFLD is due to "two hits" of steatosis followed by many different sequential steps, such as oxidative stress causing inflammation and leading to fibrosis, which in turn gives rise to cirrhosis.
