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Mapping the different sequences to the full-length SmTRC1 genomic sequence confirmed a diversity of splicing patterns, as already suggested by mapping of previously existing GenBank ESTs.
Then, in order to address relevant genetic polymorphism in the ITS multi-copy region, we assigned slightly different sequences to the representative variants, using statistical parsimony network analysis as implemented in TCS 1.21 [ 71].
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However, their siRNA targeted a different sequence to the three siRNAs used in the present study, which we tested for both specific and off-target effects.
This possibility might be controlled for by adding back different sequences to restore the length while maintaining the loss of elements specific to exon 2. Conversely, a deletion might eliminate exon skipping altogether.
Both backbone and side-chain flexibilities are needed to accomplish the structural shifts needed for the different sequences to fit into the common binding site [ 62].
We have compared the HAC-forming potential of different sequences to identify features critical to the efficiency of the process.
The binding of guide RNA is directed by its conserved secondary structure and sequence motifs, which enables guide RNA with different sequences to be incorporated into the same protein complex.
For the different sequences to bind, an "induced fit" was suggested to be required in order to accommodate the different structures of the different side chains.
The ability to attach two different siRNA sequences to the same nanoparticle would enable a single construct to simultaneously target two different mRNAs in the same cell.
Allelic variation would explain both the presence of very closely related sequences and the apparent excess of different sequences relative to the number of MSG genes in the genome.
We refer to the second most highly expressed transcript with a different protein sequence to the primary transcript as the secondary transcript.
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