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Further, each experiment was performed using a minimum of 3 different preparations for each plasmid reporter construct.
Experiments were done 3 times in triplicate with 2 different preparations for each plasmid (n = 18 for each point).
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In addition, as our study required participating laboratories to perform the assays on three separate occasions, different preparations of RBC for the replicate 3-day HA MN assays were likely.
To optimize the use of chitosan in clinical applications, it is therefore critical to address the effect of DDA on the ability of chitosan to activate PMNs and to compare different preparations of chitosan (for instance, chitosan suspensions versus scaffolds).
Two different preparations of PRMs were used for this study: (1) directly from whole blood as described above or (2) from expired platelet bags obtained through the Alfred Hospital Blood Services.
All experiments were performed in triplicate using a different preparation of F9 cells for each experiment.
The different preparations were incubated for 5 min at 37°C and then for 25 min at room temperature in the dark.
Cardiomyocytes from six different preparations were used for each treatment.
No significant differences were seen for the different preparations, and we calculated between 6.0 × 1017 and 1.3 × 1018 ligands per milligram of SIPPs.
In the current study, we compare the different preparation methods for the generation of HPV pseudovirions for their ability to efficiently infect cells.
But it added, "Caution is warranted for different preparations, and their safety should not be assumed in the absence of conclusive data".
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