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Cells from different passages of each seeding density were subjected to pluripotency genes (Oct4, Sox2, Nanog, klf4, c-Myc) and PDGFRa gene expression analysis, measurement of absolute telomere length by real-time PCR, and induction of differentiation into osteogenic, adipogenic, and chondrogenic lineages.
From different passages of each Z171 cell pool 7 × 105 cells/well were plated on 6-wells plates.
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All the values were presented as mean ± SEM, and statistically significant differences (p < 0.05) between different passages of human Sertoli cells were determined using the analysis of variance (ANOVA) and a 2-tailed t-test.
Different passages of the same fibroblast culture clustered with each other, indicating that their in vitro phenotypes were stable.
Each side pointed to different passages of the Supreme Court's ruling in a landmark 2004 case.
Sequences from different passages of the same strain were sometimes as different from each other as they were from other strains (29 ), suggesting that sequence heterogeneity observed was the result of culture adaptation, making it impossible to determine how homogeneous the pandemic viruses actually were.
We used 8 samples for each treatment group, and experiments were repeated 3 times using different passages of cells.
Different passages of DFAT cells all remained high viability (>97%) and showed no significance in each other (98.00% ± 0.82% in P5, 98.60% ± 1.14% in P29, and 99.25% ± 0.96% in P59) (p > 0.05)), suggesting that DFAT cells maintain high and stable activity during long-term culture in vitro.
To that end, he will invite six choreographers to create a dance to different passages of music by the same composer and pair them with visual artists of their choice; his wish list runs from Bartok to Bjork.
Experiments were performed in two different passages of the AZ-AHR and AZ-GR cells.
Experiments were repeated in 3 different passages of cell line.
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CEO of Professional Science Editing for Scientists @ prosciediting.com