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However, the exclusive use of regulatory matrices has limits; it requires multiple analyses with different parameters of stringency and layout of each matrix compared with the other involved regulatory elements.
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With the practical restrictions of the generalized global alignment, Huang and Brutlag describe in [19] an algorithm that computes an optimal alignment of two sequences by using a set of multiple parameters with different levels of stringency.
We find that many programs call similar peaks, though default parameters are tuned to different levels of stringency.
After trying the default parameters of the three programs, varying the stringencies of the different parameters, and manually examining the contigs, the results for Vector NTI (using more stringent parameters, i.e, overlap length cutoff of 50 and overlap percent identity of 90) were retained for further analysis.
This may suggest that informed consent bundles together several requirements with different levels of stringency.
For statistical evaluation, different levels of stringency were applied so as to reflect stringency levels commonly found in the literature [8], [14], [16].
Again, two different levels of stringency with regard to the presence of methylated DNA in plasma samples were applied.
Multiple post-hoc tests were applied in recognition of their different levels of stringency (Bonferroni > LSD), and their non-uniform popularity among statisticians.
Although limited in scope and applicability, these results suggest that different levels of stringency in peer review may accelerate or hinder scientific progress.
To determine filtering parameters used in SNP prediction that could produce suitable number of SNPs with an acceptable validation rate for fine mapping, we compared two different levels of stringency in the SNP prediction.
Two output files were constructed from the EdgeR results, based on different levels of stringency.
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