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Moreover, as indicated by the different numbers of amplification and deletion events (see Figure 3B), it is obvious that tumor samples are heterogeneous.
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For semi-quantification by RT-PCR, we first performed RT-PCR for different number of amplification cycles for each gene (Actin and Etv5 in Supplementary Figure S2).
The optimal number of cycles for each sample was determined by analyzing the PCR products of a series of PCR amplifications using different numbers of cycles by electrophoresis.
Figure 2 shows an example of real-time PCR GAPDH amplification curves using different numbers of PBMC.
The linear range of amplification for each gene transcript was determined by comparing samples obtained using different numbers of cycles.
We conducted a simulation of PCR amplification of the human genome with FR primers containing different numbers of fixed and randomized bases.
Different blocks show different numbers of haplotypes.
Different cells have different numbers of centrioles.
Provide about three different numbers of elephants.
Infections with more than one strain of P. jirovecii, which occur frequently in HIV-infected patients (20%70%% of patients) can be easily detected with this method if the different strains have a different number of repeats, since PCR amplification will result in multiple bands corresponding to the different sizes of the repeats.
Preliminary PCR amplification was carried out, using a fixed amount of cDNA template and a different number of PCR cycles, in order to determine the linear range of amplification of each product.
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