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The different levels of staining between controls and RA treated fins seem to reflect individual differences in exposure time to Alcian Blue, as the other control sample show a lighter staining.
More than half of cancer cases (59 of 93, 63.4%) scored as 0 or 1 (Table 1, upper right), indicating a reduction of H4K20me3 staining in cancer cells relative to the adjacent noncancerous regions, which stained positive and were scored as 2. A single specimen often exhibited different levels of staining between noncancerous and cancerous regions.
Among the stained nuclei, we observed different levels of staining intensity: in this effort to map DIMM expression, we focused on strong signals and did not score low-level DIMM expression (Figure S1).
In this study, three different levels of staining intensity defined as mild, moderate or strong (about 25%, 50%and75%5% of positive control) were investigated.
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The expression status of Rad51 was classified into two groups: (1) low-level expression: PCI⩽10%; (2) high-level expression: PCI >10%. Figure 1 illustrates representative examples of the different levels of Rad51 staining in SCC and ADC of NSCLC. Figure 2 gives the high order magnification picture of a SCC specimen exhibiting high-level expression of Rad51.
Figure 8A displays the different level of Hospicells staining.
We have confirmed and extended our previous analyses of prospectively isolated mouse mammary epithelial subpopulations by demonstrating that different levels of 33A10 antibody staining identify different luminal epithelial subpopulations.
Figure 1 illustrates representative examples of the different levels of YKL-40 staining in borderline ovarian tumors and OC.
Different levels of intensity of blue staining were not determined, only presence or absence of blue staining was noted.
Staining showing different levels of TCR-β expression among the described subsets are not provided, however.
No difference was noted between different levels of TNC and a slight increased intensity of the fiber staining could be detected after CFA application, but not in the gelatinous layer.
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