To evaluate the impact of different levels of HER2 mRNA on pCR in the groups of ESR1-positive and -negative tumors, we performed STEPP analysis.
This difference between Genevestigator and experimental data could be a consequence of different levels of detection in RT-PCR and microarray experiments or different experimental conditions.
In order to validate the changes in gene expression determined by RNA-seq, we selected 21 genes with different levels of transcript abundances for real-time PCR analysis.
We have described elsewhere the infection of K562 cells with different MOI units of pCCL.β-globin.PGW vector and the isolation of ten clones that show, by real-time quantitative PCR analysis, different levels of genomic integration of the human β-globin gene [ 44].
To characterize the expression profiles of the nine Rboh genes in rice seedlings during submergence, their transcript levels were determined by quantitative RT-PCR under different levels of submergence.
In order to validate the DNA microarray results before further analysis, we analyzed the m-RNA expression levels of 13 selected genes having different levels of expression by real-time quantitative RT-PCR (qPCR) in the soleus muscle, including those used for the microarray.
RT-PCR analysis showed different levels of Opn gene expression in mononucleated cells cultured in proliferation medium compared to osteoblast differentiation medium.
A total of 27 genes with different levels of expression detected using microarray were selected for real-time PCR verification using the same RNA purified for microarray hybridizations.
Telomerase activity (Fig. 3B) and hTERT mRNA (Fig. 3C) was determined to verify the changes of different levels of telomerase and hTERT gene expression by TRAP and RT-PCR respectively.
Quantitative RT-PCR analyses revealed that complex regulation of Rboh genes is involved under different levels of submergence.
There are different levels of understanding.
