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Indeed, different labs may produce different results in microarray experiments [ 30, 31], probably due to different lab protocols, analysis methods, and work patterns.
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These differences also reflect the very different nature of the two experiments, performed by different labs using different protocols.
They are typical images produced in three different labs using different protocols, which were applied to different brain areas.
It should be noted that the correlation is quite remarkable, given that the arrays were processed in different labs using different protocols.
We emphasize, that ALPS similarities should be calculated per experiment and comparisons across different datasets, generated in different labs with different protocols or even different sequencing platforms, should be performed with care.
Moreover, since the 2 Kb and 3/5 Kb libraries were produced and sequenced by different labs using different protocols it is highly unlikely that systematic technical errors have been introduced.
The Illumina platform provides users with the ability to construct application-specific sequencing libraries using a variety of lab protocols and possibly different sequencing primers.
For example, differences in measurement platforms and lab protocols can render gene expression levels incomparable.
In this work, to optimize the immunization protocol, a comparison between intragastric and intranasal routes of administration was performed and two different LAB strains (Lactococcus lactis and Lactobacillus plantarum) were tested as delivery vector.
However, the lab protocols used for both small-scale PRP preparations and larger commercial PRP preparations can yield end products with different cellular and protein (GF) and cytokine concentrations [ 20].
Cold Spring Harbor Lab Protocols (2016).
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