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Ratio of mature tenocytes and TSPC might differ using different isolation methods and could generally be a possible explanation for the differences in collagen I/decorin expression.
The number of expressed genes as well the gene expression measurements differ significantly between different isolation techniques.
The biggest differences in thickness between two different isolation materials correspond to the highest difference in the unitary costs.
It is worth noting that, as the plasmid DNA is isolated from two different microorganisms with different isolation protocols, there may be differences in the purity of plasmid DNA used for transformation, which could be a confounding factor.
This difference could be due to different isolation procedures of the membrane vesicles investigated.
These differences may be due to different isolation and expansion techniques, but also due to different donor populations [ 43– 43].
Methods to obtain lymphatic endothelial cells from several tissues [ 22– 25] have been reported and different isolation procedures have been proposed to isolate a relative pure population of pulmonary microvascular endothelial cells from humans and murine tissues [ 26– 30].
Therefore, different isolation protocols complicate the identification of differences between the two cell types.
No difference could be detected between the two different isolation methods.
To support our data, we included in the comparative analysis six whole-genome shotgun sequences of epidemiologically unrelated clinical isolates of ST-677 CC from Sweden and the United Kingdom, representing different isolation times and geographical areas.
Of five different isolation media, none affected the isolation of E. faecalis.
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