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The difference may arise from the different HER mechanism under the two conditions, e.g., there is an additional water dissociation step in the alkaline solution and graphene-based catalysts is unfavorable to facilitate this step therefore resulting in a relative high energy barrier in the whole HER process.
As differences in ligand shedding was observed between the HMEC lines that express different HER receptors, we examined levels of these ligands in cell lysates.
The HER family participates primarily in transduction of proliferation signals from various ligands, and this process involves dimer formation between different HER receptors.
The HER family participates primarily in the transduction of proliferation signal pathways from various ligands, and this process involves dimer formation between different HER receptors.
These cell lines were used to determine if expression of different HER receptors would affect secretion of selected proteins, especially the HER1 ligands that are shed and thereby further activate HER1 signaling by an autocrine process.
Cooperation between the different HER family members has been shown to contribute to carcinogenesis, both in vitro and in human cancers, and they are co-expressed in several human malignancies, including breast cancer [ 20, 34, 35].
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Competitive inhibition studies on HER-2/neu+ tumours utilizing both FRP5- and HB8696-derived scFvs (one FITC-conjugated and the other nonlabelled) could probably clarify whether these recognise the same or different HER-2/neu epitopes.
The major difficulties in interpreting these studies is that they are retrospective analyses and, in many instances, there is no satisfactory 'control' arm against which to test attributable benefit of a treatment intervention in different HER-2 subgroups.
A second interesting approach could be the study of the immunogenicity of different HER-2-positive cancer cell lines, in relation with different MHC haplotypes or with their natural as well as engineered cytokine production.
To set up the IHC procedure for BiotHER, we used cell pellet of BT474 breast cancer cells (American Type Culture Collection, Manassas, VA, USA) and a tissue array of 10 breast cancers with different HER-2 gene status (five amplified and five nonamplified tumours).
Why was it different from her hands, her lips, her cheeks, her closed eyes?
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