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Values represent average observed knockdown for RNA from different days of cell culture and were standardized to 18s rRNA expression.
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At different days of the differentiation protocol, cells were fixed with 4% PFA and stained with Oil Red O.
Cell extracts were prepared on different days of differentiation using 1× passive lysis buffer.
It can be established that CBF do not vary within cultures of different days of growth, providing conditions to perform equivalent experiments in cells grown after different days and increasing the yield of cultures obtained out of a single biopsy.
Different birds appeared on different days of the week.
Figure 5 Effect of FMNPs on HEK293 cells morphology (magnification = ×400), morphology of HEK293 cells cultured with different concentrations of FMNPs on different days, showing cells become round and floating with apoptotic characteristics.
In addition, Nucant treatment exerted a marked down regulation transcripts coding nucleolin at different days after passage of cells, whereas the expression of transcripts coding nucleophosmin was not affected.
ELISA analysis on different days after transplantation of cells into rat brain revealed that the BDNF protein expressed in brain tissues was detectable on day 1, peaked on day 7, and persisted for two weeks.
To test the reproducibility of MSIP, the analysis was performed on each of three slides assayed on different days with equal amounts of cell lysates derived from independent transfections (biological replicates).
For each of three independent experiments done on different days, a culture of A7r5 cells was trypsinized, plated on three native and three cross-linked plates, and cultured for 60 h.
These measurements (n = 18) were done in 3 different experiments on different days using different passages of cells.
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