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We also used three different constructs for the STAT3 molecules as a control as shown in Fig. 3A ii, to determine if the defective Jak-STAT signaling in the resistant replicon cell line can be overcome specifically by the modified STAT1 protein.
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Table 6 demonstrates the risk of CVD for each of the three different constructs for obesity: level of obesity (based on BMI), duration of obesity and the obese-years metric.
Using two different constructs for each caspase, we were able to downregulate the expression of caspase-3 and caspase-8 by∼80% in mature SGBS adipocytes compared with control cells overexpressing a nonsense shRNA sequence.
The ambient calculus is a concurrent calculus where the unifying notion of ambient is used to model many different constructs for distributed and mobile computation.
Our default strategy for human proteins is to design 10 15 different constructs for each protein and to attempt to crystallize each one that can be purified, in both the presence and absence of the histidine tag [9].
We designed two different constructs for AtUGE2 expression.
Three independent experiments with three different constructs for CG30420 show very similar results.
This has been referred to as the "unchurched" or the "unmosqued" movements, with a similar parallel for young Jews as well (granted, with a completely different construct for religious identity).
In our previous study [ 17], we further demonstrated that a foreign gene could be placed within the IL-60 pBlueScript IL-60 pBlueScript under IR control on a different construct for expression.
Translational was evaluated for the different constructs by normalizing the GUS protein levels with the total levels of the GUS mRNA (not normalized to PURO) as determined by Real Time RT-PCR.
Therefore, we could calculate apparent size differences for the different constructs, using the Stokes-Einstein equation [37].
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